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Humic substances as modifiers of the transdermal absorption of active phasrmaceutical ingredients
Blahuš, Petr ; Klučáková, Martina (referee) ; Sedláček, Petr (advisor)
This thesis deals with the influence of humic substances on the transdermal penetration of the analgesic drug ibuprofen. Analgesics in the form of oral administration have major side effects. Thanks to topical preparations, side effects could be reduced and also bring a number of other benefits. The problem in the development of a transdermal form of the drug is the very effective barrier capacity of the human skin. Therefore, a lot of research is focused on the search for various accelerators that can improve its properties due to interaction with the drug. In the future, such substances could also include humic acids, which have a long tradition of use in a number of therapeutic approaches, and recently examples of their use in human medicine are increasingly appearing. The research was focused on the diffusive transport of sodium ibuprofen and its acidic counterpart. Franz diffusion cells were used to simulate the real environment, and skin isolated from pig ears was used as a membrane. Using UV-VIS spectrophotometry and HPLC, the samples were analyzed. Several values were calculated from the measured data, such as the effective diffusion coefficient, which were then compared and statistically checked. The result of this thesis is data that show the positive effect of humic substances on the transport of drugs through the skin membrane.
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Membrane compartment of Can1 (MCC): specialized functional microdomain of the yeast plasma membrane
Doudová, Lenka ; Malínský, Jan (advisor) ; Vopálenská, Irena (referee)
Membrane compartment of Can1 (MCC): specialized functional microdomain of the yeast plasma membrane Yeast plasma membrane is divided into several different compartments. Membrane compartment of Can1 is specific for its protein and lipid composition, furthermore it creates furrow-like invaginations on the plasma membrane. These invaginations are made by multiprotein complexes called eisosomes, which are located in the cytosolic side of MCCs. It was established that this domain plays an important role in response to various environmental stresses. Sur7 and Nce102 are transmembrane MCC proteins of unknown function. Sur7 is most likely a structural protein within MCC, Nce102 is probably important in regulation of kinases associated with MCC/eisosomes. Key words: biological membrane, membrane microdomains, eisosome, S. cerevisiae, Sur7, Nce102
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